The Pause Stop program is a joint initiative by the Transport Accident Commission (TAC) and the Victoria Tourism Industry Council (VTIC).
The scheme uses the familiar yellow "i" signage along key regional travel routes to remind drivers to pause their journey in a bid to combat drowsiness.
Motorists will be encouraged to plan road trips before setting out and include regular stops at Visitor Information Centres, where a range of resources are available including activities for children.
The six month pilot will run until December 22 at 11 Visitor Information Centres including Bendigo and Heathcote.
VTIC CEO Felicia Mariani said driving holidays are enjoying a resurgence in Victoria and it was critical that travelers are aware of the risks when using regional roads.
"Probably that cohort of visitors is one of the most at-risk of drivers on our roads," she said.
"The chances are they're driving long distances in probably unrealistic times and on unfamiliar roads.
"They can find out more about some of the road safety messages, and also learn about what to see and do as part of their visit to the region."
Image: Tourist information signage at the Great Ocean Walk (Parks Victoria)